Plasmid
Part:BBa_K1391020:Experience
Designed by: Jing Wei "Raymond" Liu Group: iGEM14_MIT (2014-10-17)
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Applications of BBa_K1391020
This plasmid can be used for eBFP2 expression and transfection into mammalian cells.
eBFP2 is a blue fluorescent protein. It can be transfected as is as a transfection marker. With the MAV1212 backbone, it can be modified using Golden Gate Assembly to include a miRNA binding site after eBFP2. This allows eBFP2 to be repressed in the presence of targeted miRNA, and the plasmid to serve as a reporter for low concentrations of miRNA.
User Reviews
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